Broseidon
Active member
Hey there Bros and Brodettes,
so the new buzz in the bizz seems to be tissue culture cloning.
But it seems there is some gatekeeping going on with that technique at present...
Information is hard to come by and people seem to actively avoid speaking about it in too much detail.
For those of you who aren't all that familiar with this technique (like me) here is a summary of what I could gather so far:
- In tissue cloning you don't need to cut little clones (nodes) off of mother plants but instead a single leaf off of a plant can create several clones, just from the tissue culture of the leaf.
- The way it works (roughly paraphrased) is that tissue samples are taken from plants to the heart's desire (a mother that could yield say 20 or 30 healthy clones previously, can now yield hundreds of clones through tissue culture). These tissue samples are then labeled and stored in liquid nitrogen (this is the first whole I found, can't find information on how exactly they are frozen in liquid nitrogen, I doubt they are just thrown in there. Probably in plastic vials but what is in the vials, nothing? Probably some solution..).
- Once cloning is supposed to take place, the tissue samples are taken out of the liquid nitrogen and are further tampered with through reagents in a very sterile environment and then left to incubate (which reagents, how much, etc. I am unclear at the moment).
- A fully fledged clone will develop in a short time and the main advantage aside from space constraints is that all clones will be exactly the same in size, development stage etc. and there is more uniformity in the grow because of that. This has big implications on timetables etc. etc.
Now I am aware that this technique is not something for us home growers (I mean, who can realistically afford a liquid nitrogen storage solution at home?) but it is highly interesting to me nonetheless. I am always gathering information and looking to prepare for the day comes that things are legal and I might be able to start a business in the field.
And if that day ever comes, I would be highly interested in working with tissue culture cloning as I see immense advantages over classic cloning from the little information I could gather online.
So any and all information on the subject is welcome. Particularly:
- What equipment (liquid nitrogen storage, reagents, etc.) would be required and what would be a rough cost estimate to implement tissue culture cloning in a grow op
- How exactly is it carried out and what potential pitfalls are there (I saw for example that you should do the final step of applying reagents to start the clone incubation, after thawing from the liquid nitrogen storage, under a sterile hood like you find in laboratories in order to avoid contamination. Others just carry an isopropyl alcohol bottle around with them and constantly spray everything down until they are done ... Information welcome
And as always: Of course feel free to discuss anything surrounding this topic.
Stay frosty my Bros and Brodettes
Broseidon
so the new buzz in the bizz seems to be tissue culture cloning.
But it seems there is some gatekeeping going on with that technique at present...
Information is hard to come by and people seem to actively avoid speaking about it in too much detail.
For those of you who aren't all that familiar with this technique (like me) here is a summary of what I could gather so far:
- In tissue cloning you don't need to cut little clones (nodes) off of mother plants but instead a single leaf off of a plant can create several clones, just from the tissue culture of the leaf.
- The way it works (roughly paraphrased) is that tissue samples are taken from plants to the heart's desire (a mother that could yield say 20 or 30 healthy clones previously, can now yield hundreds of clones through tissue culture). These tissue samples are then labeled and stored in liquid nitrogen (this is the first whole I found, can't find information on how exactly they are frozen in liquid nitrogen, I doubt they are just thrown in there. Probably in plastic vials but what is in the vials, nothing? Probably some solution..).
- Once cloning is supposed to take place, the tissue samples are taken out of the liquid nitrogen and are further tampered with through reagents in a very sterile environment and then left to incubate (which reagents, how much, etc. I am unclear at the moment).
- A fully fledged clone will develop in a short time and the main advantage aside from space constraints is that all clones will be exactly the same in size, development stage etc. and there is more uniformity in the grow because of that. This has big implications on timetables etc. etc.
Now I am aware that this technique is not something for us home growers (I mean, who can realistically afford a liquid nitrogen storage solution at home?) but it is highly interesting to me nonetheless. I am always gathering information and looking to prepare for the day comes that things are legal and I might be able to start a business in the field.
And if that day ever comes, I would be highly interested in working with tissue culture cloning as I see immense advantages over classic cloning from the little information I could gather online.
So any and all information on the subject is welcome. Particularly:
- What equipment (liquid nitrogen storage, reagents, etc.) would be required and what would be a rough cost estimate to implement tissue culture cloning in a grow op
- How exactly is it carried out and what potential pitfalls are there (I saw for example that you should do the final step of applying reagents to start the clone incubation, after thawing from the liquid nitrogen storage, under a sterile hood like you find in laboratories in order to avoid contamination. Others just carry an isopropyl alcohol bottle around with them and constantly spray everything down until they are done ... Information welcome
And as always: Of course feel free to discuss anything surrounding this topic.
Stay frosty my Bros and Brodettes
Broseidon