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Old 12-03-2017, 09:00 PM
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blackberry blackberry is offline
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Originally Posted by PlantManBee View Post

I give up. You are still conflating the technologies. CRISPR doesn't introduce the foreign genes.
"Now going into more detail, there are two mechanisms which can occur after a DNA double strand is cleaved. Both are part of the endogenous DNA repair machinery and depend on the presence of an adequate homology repair template (see Fig. 1):

A) Non-homologous end-joining (NHEJ) happens in the absence of an exogenous homology repair template and can introduce insertions or deletions (indels) eventually leading to frameshift mutations or gene knockouts.

B) Homology-directed repair (HDR) happens if an exogenous repair template (for example, a manipulated gene) is available and can be inserted into the opened DNA strand by the repair machinery producing a precise gene modification."

If you are saying that you can use CRISPR without introducing new genes that is true, but you can use CRISPR to cleave the DNA and the own cell will use their own system to put the donor gene sequence that you want. Both things can be done with CRISPR.
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